ELISA

Enzyme-Linked Immunosorbent Assay
Antibody Capture Assay

• antigen coated onto surface of wells--> pri antibody added--> sec antibody (labelled) added
• cannot quantify

2-site Capture Assay

• antibody coated onto wells--> antigen/ hormone added--> 2nd antibody (labelled) added
• need big antigen with at least 2 possible epitopes

Competition Assay

• sec antibody coated onto wells--> defined amount of pure antigen (labelled), pri antibody and sample antigen added
• high concentration of antigen --> less colour