PCR
Typical reaction
10X buffer 5ul
dNTP 0.2mM each
MgSO4 1mM
5' primer 0.3uM
3' primer 0.3uM
DNA Genomic: 200ng/ul, Plasmid: 1-50ng, RT mixture: 2ul
KOD 1U
Water x ul
Total 50ul
Wednesday, June 20, 2007
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment