Development assay
2-5 x 10^7 cells/ ml
Wash cells in Na/K buffer
Resuspend in Na/K buffer
Do 2x serial dilution (20ul)
Spot 20ul on plate. 5 spots (original + 4 dilutions)
Take picture every 4h.
I am helping in experiments to find genes that regulate growth of E.coli. End aim: improve yield of plasmids from E.coli and improve growth of E.coli. This blog contains what I've done, learnt, and things which I should read, and some of my ideas.
2-5 x 10^7 cells/ ml
Wash cells in Na/K buffer
Resuspend in Na/K buffer
Do 2x serial dilution (20ul)
Spot 20ul on plate. 5 spots (original + 4 dilutions)
Take picture every 4h.
at
1:36 PM
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