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I am helping in experiments to find genes that regulate growth of E.coli. End aim: improve yield of plasmids from E.coli and improve growth of E.coli. This blog contains what I've done, learnt, and things which I should read, and some of my ideas.

Thursday, July 24, 2008

Western Blot

Protein concentration assay
http://www.bio-rad.com/LifeScience/pdf/Bulletin_9005.pdf

Western transfer apparatus
http://www.bio-rad.com/LifeScience/pdf/Bulletin_9002.pdf

myc antibody (mouse)
https://www.roche-applied-science.com/pack-insert/1667149a.pdf
65kDa

bim antibody (rabbit)
http://www.bdbiosciences.com/external_files/pm/doc/tds/cell_bio/live/web_enabled/68871N_559685.pdf 23kDa

EZH2 antibody (mouse)
http://www.cellsignal.com/products/3147.html
98kDa

at 2:29 PM

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