Immunostaining

Thaw and air dry sections ~3h
Wash 2x 10min in PBS
Block 1.5h RT with blocking solution (1ml/ slide)
Incubate anti-Vangl2 Ab 1:1000 O/N 4C (500ul/ slide)
Wash 5x 10min PBS
Block 1.5h RT
Incubate secondary Ab anti-gp 488 (1:250) O/N 4C
Wash 5x 10min PBS (dark)
Mount in Miowol (100ul)

Blocking buffer (50ml)
10% Goat serum 5ml
1% BSA 0.5g
in PBS or PBT (0.1% Triton-X)

Antibody solution buffer (50ml)
1% Goat serum 0.5ml
1% BSA 0.5g
in PBS or PBT