Professor from Korea visited. Presentation by Dave on his phD proj, got an idea what he's doing. Main aim is to find an industrial way to make E.coli cells produce a lot of plasmids containing gene of interest (DNA vaccine) by overexpressing some genes and knocking out others. Plasmid containing cells will grow slower than plasmid free ones..

Streaked bacteria on agar. Must remember to turn loop around when streaking another direction, and change loop after that. Do everything inside the bio cabinet, and away fr the air drain thing. Make sure petri, loops don't touch anything other than the agar and bacteria. Throw all apparatus that touched bacteria into yellow bags.
1. Take bacteria stock from freezer, labelled agar plates from cold room, loops into cabinet
2. Spray gloves with alcohol, wash and dry
3. Dip loop inside bacteria stock, streak on one edge of dish without touching edge of dish, turn loop, streak 3 streaks in another direction, throw loop
4. change loop and change direction and streak again to dilute, change direction again and streak
5. Put plates in 37 degrees oven to incubate overnight
6. Clean up cabinet with alcohol, put all equipment back to place
7. Throw gloves, wash hands with soap

Had huge discussion about pair-wise comparison and clustering. Haven't understood yet.