Miniprep on run IV samples

Glucose conc analysis.
Using a YSI something machine. Basically it works by sucking up some sample (25-80 ul) using the sipper, which is a tiny tube, then putting the sample into some buffer, which dilutes the sample. Then this buffer + sample is passed through a 3 membranes. First is a oh no, can't remember, think it's something that don't allow molecules larger than glucose to pass through. Second is an immobilised layer of glucose oxidase, familiar? Third is a membrane made of cellulose acetate I think, it only allows H2O2 to pass through, no, actually it doesn't allow molecules bigger than Mr 40 to pass through. Then H2O2 passes to a platinum anode which oxidises H2O2 to O2 + e- + H+.. Basically produce electrons, and then there's a silver cathode. Then machine measures max current which is directly proportional to no. of electrons produced, which is proportional to amount of H2O2 formed which is proportional to amount of glucose. Yay.

That's what I've learnt during the 4h spent feeding the machine with my samples.. Super slow machine.

The machine can also measure lactose conc, sucrose conc, maltsomething conc, NH4+ conc, K+ conc. Basically for all the sugars, there's some enzyme on the membrane that converts them to H2O2 and blah, measure current. For the ions, measure the potential differenc between 2 sidees of the membrane

Attempted to use the HPLC machine, whatever it stands for, basically it's supposed to quantify amount of DNA in a sample. Anyway, results turned out really weird, so let's just pretend that we've never used it.

Dave measured biomass. Dry/ bake samples in 60 degrees oven for as long as possible. Smash all the biomass out of the tube and measure its mass.