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I am helping in experiments to find genes that regulate growth of E.coli. End aim: improve yield of plasmids from E.coli and improve growth of E.coli. This blog contains what I've done, learnt, and things which I should read, and some of my ideas.

Thursday, March 31, 2005

Glycogen test on bacteria

Parrou J L, François J. A simplified procedure for a rapid and reliable assay of both glycogen and trehalose in whole yeast cells. Anal Biochem 1997;248:186–188.

Preiss J. Bacterial glycogen synthesis and its regulation. Annu Rev Microbiol 1984;38:419–458

Aimee E. Belanger, Graham F. Hatfull Exponential-Phase Glycogen Recycling Is Essential for Growth of Mycobacterium smegmatis. J Bacteriol. 1999 November; 181(21): 6670–6678.

CE.Gallagher, M.Cervantes-Vervantes, E.T.Wurtzel Surrogate biochemistry: Use of Eschrichia coli to identify plant cDNAs that impact metabolic engineering of carotenoid accumulation. Appl Microbiol Biotechnol (2003) 60:713-719

at 3:22 PM

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      • Glycogen test on bacteria
      • Glycobiology
      • Test for glycogen
      • Bacterial Growth: Page 17/30
      • Expts to do..1. Plasmid loss in LB over time (2 we...
      • Growth rate
      • M9 monitoring, slow growth. Lag phase was 6h in sh...
      • Microarray troubleshooting: Viewed the slides, bet...
      • I am done with acetic acid assays.. for the time b...
      • Microarray
      • Transforming Bacteria
      • So long nv update. Here's what I've been doing and...
      • Acetic acid assayUV method (wavelength: 340nm)Some...
      • Blue white selection
      • Miniprep on Run V. Quantified amount of plasmid at...
      • Miniprep on run IV samplesGlucose conc analysis.Us...
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