Finally did something interesting, but quite screwed up

Suspect microarray chips are faulty
Aim of expt: Confirm that microarray chips are faulty using positive control
1. Use control cDNA (Ara? dna labelled with Cy5)
2. Follow protocol of hybridisation and hybridise onto suspected faulty chips
3. Faulty chips: from previous bioreactor run, put 1 slide in boiling water for 3 min to denature and remove DNA bound to slide, dry, use other slide as it is cos supposedly the Ara? cDNA will bind to the non-E.coli spots which will be empty
4. Paste all the stickers properly and lower coverslip gently onto 160ul of cDNA + buffer on slide, try not to create bubbles
5. Incubate overnight at 42degrees
6. Run gel with cDNA and scan with Typhoon

Screw ups
1. Left slide 1 in water and left it to heat up and boil instead of put in when the water is boiling
2. When putting down coverslip on slide 2, major screw up, cDNA slipped out of the frame, so I added more cDNA fr the original tube, the one w/o treatment onto the slide, hope it works
3. Didn't cover up water bath
4. Didn't cover up gel, but that's ok cos the gel looks fine